{"id":846,"date":"2014-06-02T13:39:00","date_gmt":"2014-06-02T06:39:00","guid":{"rendered":"https:\/\/beta.cbbiotec.vn\/?p=846"},"modified":"2025-07-01T13:16:15","modified_gmt":"2025-07-01T06:16:15","slug":"lop-tap-huan-ki-thuat-di-truyen-co-ban-khoa-i","status":"publish","type":"post","link":"https:\/\/cbbiotec.vn\/en\/lop-tap-huan-ki-thuat-di-truyen-co-ban-khoa-i\/","title":{"rendered":"L\u1edaP T\u1eacP HU\u1ea4N \u201cK\u0128 THU\u1eacT DI TRUY\u1ec0N C\u01a0 B\u1ea2N\u201d \u2013 KH\u00d3A I"},"content":{"rendered":"

Nh\u1eb1m trang b\u1ecb c\u00e1c ki\u1ebfn th\u1ee9c c\u01a1 b\u1ea3n v\u1ec1 Di truy\u1ec1n cho c\u00e1c c\u00e1 nh\u00e2n v\u00e0 doanh nghi\u1ec7p ho\u1ea1t \u0111\u1ed9ng trong l\u0129nh v\u1ef1c Sinh h\u1ecdc, Trung t\u00e2m Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 sinh h\u1ecdc v\u1eeba qua \u0111\u00e3 t\u1ed5 ch\u1ee9c th\u00e0nh c\u00f4ng l\u1edbp t\u1eadp hu\u1ea5n \u201cK\u0129 thu\u1eadt Di truy\u1ec1n c\u01a1 b\u1ea3n\u201d \u2013 kh\u00f3a I v\u1ec1 k\u1ef9 thu\u1eadt t\u1ea1o d\u00f2ng.<\/p>\n\n\n\n

\"\"
C\u00e1c h\u1ecdc vi\u00ean \u0111\u1ea7u ti\u00ean ho\u00e0n th\u00e0nh kho\u00e1 h\u1ecdc<\/mark><\/figcaption><\/figure>\n\n\n\n

<\/p>\n\n\n\n

Kh\u00f3a h\u1ecdc k\u00e9o d\u00e0i trong 4 tu\u1ea7n (t\u1eeb 10\/05\/2014 \u0111\u1ebfn 01\/06\/2014), c\u00e1c ki\u1ebfn th\u1ee9c l\u00fd thuy\u1ebft v\u00e0 th\u1ef1c h\u00e0nh \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh song song theo t\u1eebng n\u1ed9i dung chi ti\u1ebft.<\/p>\n\n\n\n\n\n\n\n

  • K\u1ef9 n\u0103ng chung \u0111\u1ec3 c\u00f3 th\u1ec3 l\u00e0m vi\u1ec7c hi\u1ec7u qu\u1ea3 trong ph\u00f2ng th\u00ed nghi\u1ec7m: Quy t\u1eafc ph\u00f2ng th\u00ed nghi\u1ec7m, b\u1ed1 tr\u00ed th\u00ed nghi\u1ec7m, s\u1eafp x\u1ebfp m\u1eabu, c\u00e1ch ghi ch\u00fa, b\u1ea3o qu\u1ea3n m\u1eabu,\u2026<\/li>
  • L\u00fd thuy\u1ebft v\u1ec1 k\u1ef9 thu\u1eadt t\u1ea1o d\u00f2ng<\/li>
  • Thi\u1ebft k\u1ebf m\u1ed3i tr\u00ean ch\u01b0\u01a1ng tr\u00ecnh m\u00e1y t\u00ednh<\/li>
  • Khu\u1ebfch \u0111\u1ea1i gen b\u1eb1ng k\u1ef9 thu\u1eadt PCR<\/li>
  • Ki\u1ec3m tra s\u1ea3n ph\u1ea9m PCR b\u1eb1ng k\u1ef9 thu\u1eadt \u0111i\u1ec7n di tr\u00ean gel agarose<\/li>
  • Tinh s\u1ea1ch s\u1ea3n ph\u1ea9m PCR b\u1eb1ng b\u1ed9 kit<\/li>
  • C\u1eaft plasmid v\u00e0 s\u1ea3n ph\u1ea9m PCR \u0111\u00e3 \u0111\u01b0\u1ee3c tinh ch\u1ebf b\u1eb1ng enzyme c\u1eaft h\u1ea1n ch\u1ebf<\/li>
  • Tinh s\u1ea1ch s\u1ea3n ph\u1ea9m sau khi c\u1eaft b\u1eb1ng b\u1ed9 kit<\/li>
  • N\u1ed1i gen v\u00e0o plasmid<\/li>
  • Bi\u1ebfn n\u1ea1p s\u1ea3n ph\u1ea9m n\u1ed1i v\u00e0o t\u1ebf b\u00e0o E.coli<\/li>
  • S\u00e0ng l\u1ecdc d\u00f2ng t\u1ebf b\u00e0o mang plasmid t\u00e1i t\u1ed5 h\u1ee3p b\u1eb1ng PCR khu\u1ea9n l\u1ea1c<\/li>
  • T\u00e1ch chi\u1ebft plasmid<\/li>
  • C\u1eaft ki\u1ec3m tra plasmid<\/li>
  • Gi\u1ea3i tr\u00ecnh t\u1ef1 v\u00e0 ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 gi\u1ea3i tr\u00ecnh t\u1ef1<\/li><\/ul>\n\n\n\n\n\n

    Sau kh\u00f3a h\u1ecdc, c\u00e1c h\u1ecdc vi\u00ean \u0111\u00e3 c\u00f3 th\u1ec3 n\u1eafm v\u1eefng \u0111\u01b0\u1ee3c c\u00e1c k\u0129 thu\u1eadt c\u01a1 b\u1ea3n v\u1ec1 thao t\u00e1c th\u1ef1c nghi\u1ec7m trong Di truy\u1ec1n ph\u00e2n t\u1eed c\u0169ng nh\u01b0 nh\u1eefng ki\u1ebfn th\u1ee9c n\u1ec1n t\u1ea3ng c\u1ea7n c\u00f3 \u0111\u1ec3 ph\u1ee5c v\u1ee5 cho qu\u00e1 tr\u00ecnh c\u00f4ng t\u00e1c v\u00e0 nghi\u00ean c\u1ee9u khoa h\u1ecdc sau n\u00e0y.<\/p>\n\n\n\n

    \"\"<\/figure>
    \n

    Gi\u1ea3ng vi\u00ean h\u01b0\u1edbng d\u1eabn<\/strong><\/p>\n\n\n\n

    PGS. TS. Phan Th\u1ecb Ph\u01b0\u1ee3ng Trang \u2013 Gi\u1ea3ng vi\u00ean tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc Khoa h\u1ecdc t\u1ef1 nhi\u00ean TPHCM, Tr\u01b0\u1edfng nh\u00f3m nghi\u00ean c\u1ee9u thu\u1ed9c Trung t\u00e2m Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc. <\/p>\n<\/div><\/div>\n\n\n\n

    <\/p>","protected":false},"excerpt":{"rendered":"

    Nh\u1eb1m trang b\u1ecb c\u00e1c ki\u1ebfn th\u1ee9c c\u01a1 b\u1ea3n v\u1ec1 Di truy\u1ec1n cho c\u00e1c c\u00e1 nh\u00e2n v\u00e0 doanh nghi\u1ec7p ho\u1ea1t \u0111\u1ed9ng trong l\u0129nh v\u1ef1c Sinh h\u1ecdc, Trung t\u00e2m Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 sinh h\u1ecdc v\u1eeba […]<\/p>","protected":false},"author":7,"featured_media":859,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[97],"tags":[],"class_list":["post-846","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-activities"],"_links":{"self":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts\/846","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/comments?post=846"}],"version-history":[{"count":11,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts\/846\/revisions"}],"predecessor-version":[{"id":865,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts\/846\/revisions\/865"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/media\/859"}],"wp:attachment":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/media?parent=846"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/categories?post=846"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/tags?post=846"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}