{"id":554,"date":"2022-03-25T16:20:45","date_gmt":"2022-03-25T09:20:45","guid":{"rendered":"https:\/\/beta.cbbiotec.vn\/?p=554"},"modified":"2025-07-01T13:16:03","modified_gmt":"2025-07-01T06:16:03","slug":"production-of-recombinant-protein","status":"publish","type":"post","link":"https:\/\/cbbiotec.vn\/en\/production-of-recombinant-protein\/","title":{"rendered":"Production of Recombinant protein"},"content":{"rendered":"<p style=\"text-align: justify;\">Tr\u00ean th\u1ebf gi\u1edbi, theo th\u1ed1ng k\u00ea n\u0103m 2016, th\u1ecb tr\u01b0\u1eddng protein t\u00e1i t\u1ed5 h\u1ee3p \u0111\u00e3 \u0111\u1ea1t gi\u00e1 tr\u1ecb 347,2 tri\u1ec7u \u0111\u00f4 la M\u1ef9 v\u00e0 \u01b0\u1edbc t\u00ednh gi\u00e1 tr\u1ecb n\u00e0y s\u1ebd v\u01b0\u1ee3t ng\u01b0\u1ee1ng 593,4 tri\u1ec7u \u0111\u00f4 la M\u1ef9 v\u00e0o n\u0103m 2025. H\u1ea7u h\u1ebft c\u00e1c s\u1ea3n ph\u1ea9m protein t\u00e1i t\u1ed5 h\u1ee3p \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u00e0m d\u01b0\u1ee3c ph\u1ea9m nh\u01b0 G-CSF, Erythropoietin, Interferon, Vaccines\u2026 gi\u00fap ph\u00f2ng ng\u1eeba, ch\u1ea9n \u0111o\u00e1n v\u00e0 \u0111i\u1ec1u tr\u1ecb c\u00e1c b\u1ec7nh ti\u1ec3u \u0111\u01b0\u1eddng, ung th\u01b0, thi\u1ebfu m\u00e1u&#8230; Ngo\u00e0i ra, c\u00e1c enzyme t\u00e1i t\u1ed5 h\u1ee3p nh\u01b0 protease, amylase, lipase, cellulase, pullulanase v\u00e0 pectinase\u2026\u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng r\u1ed9ng r\u00e3i trong s\u1ea3n xu\u1ea5t v\u00e0 ch\u1ebf bi\u1ebfn th\u1ef1c ph\u1ea9m (bia, r\u01b0\u1ee3u, n\u01b0\u1edbc ch\u1ea5m, n\u01b0\u1edbc gi\u1ea3i kh\u00e1t, th\u1ecbt, c\u00e1 v\u00e0 c\u00e1c n\u00f4ng l\u00e2m th\u1ee7y h\u1ea3i s\u1ea3n kh\u00e1c\u2026) gi\u00fap \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh c\u1ee7a ch\u1ea5t l\u01b0\u1ee3ng s\u1ea3n ph\u1ea9m, t\u0103ng s\u1ee9c c\u1ea1nh tranh tr\u00ean th\u1ecb tr\u01b0\u1eddng th\u1ebf gi\u1edbi. T\u00ednh \u0111\u1ebfn n\u0103m 2015, doanh thu c\u1ee7a ri\u00eang c\u00e1c s\u1ea3n ph\u1ea9m enzyme c\u00f4ng nghi\u1ec7p n\u00e0y \u0111\u1ea1t 3,74 t\u1ef7 USD. Ch\u00ednh v\u00ec v\u00e2y, vi\u1ec7c nghi\u00ean c\u1ee9u nh\u1eb1m bi\u1ec3u hi\u1ec7n v\u00e0 tinh ch\u1ebf \u0111\u1ec3 thu nh\u1eadn c\u00e1c protein\/ enzyme c\u00f3 \u0111\u1ed9 tinh s\u1ea1ch cao nh\u1eb1m ph\u1ee5c v\u1ee5 cho c\u00e1c nghi\u00ean c\u1ee9u c\u01a1 b\u1ea3n va \u1ee9ng d\u1ee5ng s\u1ea3n xu\u1ea5t l\u00e0 th\u1eadt s\u1ef1 c\u1ea7n thi\u1ebft.<\/p>\n<p style=\"text-align: justify;\">\u0110\u1ec3 bi\u1ec3u hi\u1ec7n c\u00e1c protein t\u00e1i t\u1ed5 h\u1ee3p, nh\u00f3m ch\u00fang t\u00f4i s\u1eed d\u1ee5ng c\u00e1c h\u1ec7 th\u1ed1ng bi\u1ec3u hi\u1ec7n cho B. subtilis \u0111\u01b0\u1ee3c t\u1ea1o ra b\u1edfi nh\u00f3m nghi\u00ean c\u1ee9u, hay <em>E. coli<\/em> v\u00e0 n\u1ea5m men t\u1eeb c\u00e1c h\u1ec7 th\u1ed1ng bi\u1ec3u hi\u1ec7n th\u01b0\u01a1ng m\u1ea1i. Ngo\u00e0i ra, m\u1ed9t h\u01b0\u1edbng nghi\u00ean c\u1ee9u kh\u00e1c c\u1ee7a nh\u00f3m l\u00e0 t\u1ea1o ra c\u00e1c ch\u1ee7ng vi sinh v\u1eadt c\u00f3 ti\u1ec1m n\u0103ng bi\u1ec3u hi\u1ec7n c\u00e1c enzyme th\u1ee7y ph\u00e2n cellulose, tinh b\u1ed9t, chitin, protein, lipid\u2026 \u0111\u1eb7c bi\u1ec7t l\u00e0 l\u00e0 c\u00e1c ch\u1ee7ng vi sinh v\u1eadt c\u00f3 kh\u1ea3 n\u0103ng \u0111\u1ed1i kh\u00e1ng v\u1edbi c\u00e1c vi sinh v\u1eadt g\u00e2y b\u1ec7nh. C\u00e1c ch\u1ee7ng vi sinh v\u1eadt n\u00e0y c\u00f3 ti\u1ec1m n\u0103ng \u1ee9ng d\u1ee5ng trong s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m vi sinh ph\u1ee5c v\u1ee5 cho vi\u1ec7c x\u1eed l\u00fd r\u00e1c th\u1ea3i, n\u01b0\u1edbc th\u1ea3i, n\u01b0\u1edbc trong nu\u00f4i tr\u1ed3ng, ph\u00e2n b\u00f3n, thu\u1ed1c tr\u1eeb s\u00e2u\u2026<\/p>\n<p style=\"text-align: justify;\">Nh\u00f3m nghi\u00ean c\u1ee9u \u0111\u01b0\u1ee3c d\u1eabn d\u1eaft tr\u1ef1c ti\u1ebfp b\u1edfi PGS.TS. Nguy\u1ec5n \u0110\u1ee9c Ho\u00e0ng c\u00f9ng v\u1edbi s\u1ef1 h\u1ed7 tr\u1ee3 c\u1ee7a PGS.TS. Phan Th\u1ecb Ph\u01b0\u1ee3ng Trang v\u00e0 GS. Wolfgang Schumann.<\/p>\n\n\n\n\n<p style=\"text-align: justify;\">Hi\u1ec7n nay, h\u01b0\u1edbng bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p nh\u1eb1m t\u1ea1o ra c\u00e1c protein ph\u1ee5c v\u1ee5 cho nghi\u00ean c\u1ee9u, s\u00e0ng l\u1ecdc thu\u1ed1c, s\u1ea3n xu\u1ea5t kh\u00e1ng nguy\u00ean v\u00e0 kh\u00e1ng th\u1ec3, c\u00e1c enzyme d\u00f9ng trong sinh h\u1ecdc ph\u00e2n t\u1eed v\u00e0 trong c\u00f4ng nghi\u1ec7p, nh\u00f3m nghi\u00ean c\u1ee9u \u0111ang t\u1eadp trung ch\u1ee7 y\u1ebfu v\u00e0o 3 h\u01b0\u1edbng ch\u00ednh:<\/p>\n<ul>\n<li style=\"text-align: justify;\">Bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p \u1edf d\u1ea1ng n\u1ed9i b\u00e0o<\/li>\n<li style=\"text-align: justify;\">Bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p \u1edf d\u1ea1ng ngo\u1ea1i b\u00e0o&nbsp;<\/li>\n<li style=\"text-align: justify;\">C\u00f4ng ngh\u1ec7 bi\u1ec3u hi\u1ec7n b\u1ec1 m\u1eb7t<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">&#8211; Bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p \u1edf d\u1ea1ng n\u1ed9i b\u00e0o nh\u1eb1m t\u1ea1o ra c\u00e1c protein ph\u1ee5c v\u1ee5 cho nghi\u00ean c\u1ee9u, s\u00e0ng l\u1ecdc thu\u1ed1c, s\u1ea3n xu\u1ea5t kh\u00e1ng nguy\u00ean v\u00e0 kh\u00e1ng th\u1ec3, c\u00e1c enzyme d\u00f9ng trong sinh h\u1ecdc ph\u00e2n t\u1eed v\u00e0 trong c\u00f4ng nghi\u1ec7p. V\u00ed d\u1ee5 nh\u01b0 c\u00e1c protein marker ph\u00e1t hu\u1ef3nh quang (GFP, CFP, YFP\u2026), Taq DNA polymerase, TEV, HRV3C, \u03b2-galactosidase, Kinase, nuclear receptor, LLO, IntA, LTB, VP19, VP28\u2026<\/p>\n<p style=\"text-align: justify;\">&#8211; Bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p \u1edf d\u1ea1ng ngo\u1ea1i b\u00e0o nh\u1eb1m t\u1ea1o ra c\u00e1c protein ph\u1ee5c v\u1ee5 cho nghi\u00ean c\u1ee9u, v\u00e0 s\u1ea3n xu\u1ea5t. V\u00ed d\u1ee5 nh\u01b0 cellulase, lipase, natokinase, amylase, protease,\u2026<\/p>\n<p style=\"text-align: justify;\">&#8211; Bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o nh\u1eb1m t\u1ea1o ra c\u00e1c ch\u1ee7ng vi sinh v\u1eadt c\u00f3 kh\u1ea3 n\u0103ng bi\u1ec3u hi\u1ec7n c\u00e1c protein tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o nh\u1eb1m \u1ee9ng d\u1ee5ng trong nghi\u00ean c\u1ee9u v\u00e0 s\u1ea3n xu\u1ea5t vaccine, probiotic, ch\u1ebf ph\u1ea9m vi sinh,\u2026.<\/p>\n<p>C\u00e1c protein sau bi\u1ec3u hi\u1ec7n \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh tinh ch\u1ebf d\u1ef1a tr\u00ean c\u00e1c li\u00ean k\u1ebft \u00e1i l\u1ef1c c\u1ee7a \u0111u\u00f4i dung h\u1ee3p (His tag, GST tag, Strep tag,\u2026) v\u1edbi c\u00e1c nh\u00e2n t\u1ed1 kh\u00e1c (Ni-NTA, Glutathione, Strep-Tactin\u2026) nh\u1eb1m thu nh\u1eadn protein m\u1ee5c ti\u00eau c\u00f3 \u0111\u1ed9 tinh s\u1ea1ch cao \u0111\u1ec3 \u0111\u00e1p \u1ee9ng c\u00e1c nhu c\u1ea7u trong nghi\u00ean c\u1ee9u c\u01a1 b\u1ea3n c\u0169ng nh\u01b0 trong \u1ee9ng d\u1ee5ng \u1edf c\u00e1c l\u0129nh v\u1ef1c y d\u01b0\u1ee3c, c\u00f4ng nghi\u1ec7p, th\u1ef1c ph\u1ea9m,&#8230;<\/p>\n\n\n\n<figure class=\"wp-block-image is-style-default\"><img decoding=\"async\" src=\"http:\/\/cbbiotec.vn\/images\/sampledata\/recombinantprotein\/1.jpg\" alt=\"\"\/><figcaption class=\"wp-element-caption\"><em>Bi\u1ec3u hi\u1ec7n v\u00e0 tinh ch\u1ebf protein cSRC d\u1ea1ng dung h\u1ee3p v\u00e0 lo\u1ea1i b\u1ecf \u0111u\u00f4i dung h\u1ee3p b\u1eb1ng TEV<\/em><\/figcaption><\/figure>\n\n\n\n\n\n\n\n<p style=\"text-align: justify;\"><em>B. subtilis<\/em> \u0111\u01b0\u1ee3c coi l\u00e0 vi khu\u1ea9n m\u00f4 h\u00ecnh cho c\u00e1c ch\u1ee7ng vi khu\u1ea9n Gram d\u01b0\u01a1ng v\u1edbi c\u00e1c \u01b0u \u0111i\u1ec3m \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn nh\u01b0: (1) Kh\u00f4ng c\u00f3 endotoxin (2) H\u1ec7 th\u1ed1ng plasmid t\u1ed3n t\u1ea1i \u0111\u1ed9c l\u1eadp cho ph\u00e9p bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p tr\u00ean ch\u1ee7ng ch\u1ee7 <em>B. subtilis<\/em> \u1edf c\u1ea3 d\u1ea1ng ti\u1ebft ra m\u00f4i tr\u01b0\u1eddng ho\u1eb7c n\u1ed9i b\u00e0o \u1edf m\u1ee9c cao. (3) M\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y r\u1ebb ti\u1ec1n, quy tr\u00ecnh nu\u00f4i c\u1ea5y \u0111\u01a1n gi\u1ea3n v\u1edbi chi ph\u00ed th\u1ea5p nh\u01b0ng hi\u1ec7u qu\u1ea3 t\u1ea1o s\u1ea3n ph\u1ea9m, t\u1ea1o sinh kh\u1ed1i cao (4) B. subtilis \u0111\u01b0\u1ee3c c\u00f4ng nh\u1eadn l\u00e0 GRAS \u2013 General Regarded As Safe, b\u1edfi FDA \u2013 Foods and Drugs Administration, M\u1ef9. Do \u0111\u00f3, B. subtilis l\u00e0 ch\u1ee7ng ch\u1ee7 ti\u1ec1m n\u0103ng trong s\u1ea3n xu\u1ea5t protein t\u00e1i t\u1ed5 h\u1ee3p.<\/p>\n<p style=\"text-align: justify;\">Ngo\u00e0i ra nh\u00f3m nghi\u00ean c\u1ee9u c\u00f2n c\u00f3 kinh nghi\u1ec7m trong vi\u1ec7c thi\u1ebft k\u1ebf v\u00e0 l\u1ef1a ch\u1ecdn h\u1ec7 th\u1ed1ng bi\u1ec3u hi\u1ec7n hi\u1ec7u qu\u1ea3 cho <em>B. subtilis<\/em> n\u00ean vi\u1ec7c th\u00e0nh c\u00f4ng trong vi\u1ec7c bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p tr\u00ean <em>B. subtilis<\/em> l\u00e0 ho\u00e0n to\u00e0n c\u00f3 ti\u1ec1m n\u0103ng. Ngo\u00e0i ra, v\u1edbi \u0111\u1ed9i ng\u0169 nhi\u1ec1u kinh nghi\u1ec7m trong t\u1ed1i \u01b0u h\u00f3a gen t\u1eeb nh\u00f3m Tin \u2013 Sinh h\u1ecdc, ch\u00fang t\u00f4i d\u1ec5 d\u00e0ng s\u1eed d\u1ee5ng c\u00e1c c\u00f4ng c\u1ee5 v\u1ec1 Tin \u2013 Sinh h\u1ecdc \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a gen m\u1ee5c ti\u00eau, t\u1ed1i \u01b0u h\u00f3a vi\u1ec7c bi\u1ec3u hi\u1ec7n ngay t\u1eeb qu\u00e1 tr\u00ecnh thi\u1ebft k\u1ebf gen v\u00e0 plasmid t\u00e1i t\u1ed5 h\u1ee3p. \u0110\u00e2y l\u00e0 y\u1ebfu t\u1ed1 g\u00f3p ph\u1ea7n cho th\u00e0nh c\u00f4ng trong vi\u1ec7c bi\u1ec3u hi\u1ec7n protein t\u00e1i t\u1ed5 h\u1ee3p tr\u00ean <em>B. subtilis.<\/em><\/p>","protected":false},"excerpt":{"rendered":"<p>Tr\u00ean th\u1ebf gi\u1edbi, theo th\u1ed1ng k\u00ea n\u0103m 2016, th\u1ecb tr\u01b0\u1eddng protein t\u00e1i t\u1ed5 h\u1ee3p \u0111\u00e3 \u0111\u1ea1t gi\u00e1 tr\u1ecb 347,2 tri\u1ec7u \u0111\u00f4 la M\u1ef9 v\u00e0 \u01b0\u1edbc t\u00ednh gi\u00e1 tr\u1ecb n\u00e0y s\u1ebd v\u01b0\u1ee3t ng\u01b0\u1ee1ng 593,4 tri\u1ec7u \u0111\u00f4 [&hellip;]<\/p>","protected":false},"author":7,"featured_media":1923,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57],"tags":[],"class_list":["post-554","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research"],"_links":{"self":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts\/554","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/comments?post=554"}],"version-history":[{"count":23,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts\/554\/revisions"}],"predecessor-version":[{"id":1992,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/posts\/554\/revisions\/1992"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/media\/1923"}],"wp:attachment":[{"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/media?parent=554"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/categories?post=554"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cbbiotec.vn\/en\/wp-json\/wp\/v2\/tags?post=554"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}